A drosophila chamber’s primary objective is to create an artificial environment for the growth and processes involving a drosophila melanogaster. A drosophila melanogaster is a species of fly in the Drosophilidae family. The species is known generally as the common fruit fly or vinegar fly. D. melanogaster continues to be widely used for biological research in studies of genetics, physiology, microbial pathogenesis, and life history evolution. The melanogaster is used because it is an insect that thrives on ease of accessibility and care; it has four pairs of chromosomes, breeds quickly and lays a lot of eggs.
Drosophila incubation is the most operated feature in the drosophila chamber; it is the main objective to be precise. The process of drosophila incubation is relatively long and time consuming; most steps in the process are important. The most important aspects are the final pH of the medium, the level of compression of the egg chamber and the quality of the dissected egg chamber. The amount of light exposure into the chamber is also important.
Equipments needed for the Drosophila Incubation:
• Dissecting microscope
• 0.5ml micro centrifuge tubes
• Two pairs of sharp forceps (Dumont, no. 5)
• Two-well depression glass slides (Fisher Scientific, catalog number 12 565B)
• Greiner Lumox™ culture dish Hydrophilic (50mm) (Sigma catalog number Z376744)
• Fisher brand No. 1 coverslips (catalog number 12-542-B)
• Imaging microscope and software (for example, Axiovision from Zeiss)
• pH Meter
• Heat filter KG1 (Chroma technologies)
• BG38 IR suppression filter (Chroma technologies)
• Neutral density filters (ND 0.3) (Chroma technologies)
The drosophila chamber also possesses the ability to act as an incubator. It helps with the process of insect incubation based on the eggs laid. It creates an artificial environment that keeps the egg at the right temperature, as derived by the scientist, which helps the egg to incubate the organism inside it and cultivate it into a living being.
Generic cabinet construction of a Drosophila Chamber:
• Interior constructed of 22-gauge electro-zinc plated steel.
• Exterior constructed of 18-gauge exterior electro-zinc plated steel.
• Welded seams and joints on outer and inner shells.
• Insulation: Foamed in place rigid polyurethane.
• Chamber is completely self-contained.
• Overall wall thickness is 2”.
• One 1 1/4” diameter access port on R.H. wall.
• Chamber floor equipped with floor drain.
• Contains caster wheels and adjustable leveling legs to balance out for floor unevenness in the lab.
• Door with magnetic gasket & opening provides full access to chamber interior.
• Six tiers of white epoxy coated steel wire shelving (each shelf is 27”D x 28.7”W).
• Shelves are supported by shelf clips allowing 1/2” vertical adjustments.
• Interior and exterior painted with high reflective, environmentally friendly, high temperature baked white powder coating.
The right research is required before the purchase of a drosophila chamber; there are a lot of factors that affect the choice of the chamber such as the experiment to be conducted, the type of the insect or egg and many more. Obtain a detailed description of the drosophila chamber, Inquire here!